Table 1: Mortality of Larvae at Different Concentrations of Neem Seed Oil and Different Time

Petri dishes (9 cm diameter) with one sheet of Þ lter paper inside of the same size were used for the experiment. Five different concentrations of neem oil, 100, 80, 60, 40 and 20% were used for the study. One milliliter (100%), 0.8 ml (80%), 0.6 ml (60%), 0.4 ml (40%) and 0.2 ml (20%) of neem oil were evenly soaked (spread with a spatula) on different filter papers of each Petri dish. Distilled water soaked in filter paper was used as a control (0% concentration). Then different numbers of larvae were put inside each plate with the help of a soft brush and then monitored at certain interval of time. The number of larvae found dead in each plate was recorded and the results were summarized in Table 1.